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Islet proteins were extracted using RIPA buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 1% sodium deoxycholate, 5 mM EDTA, 1 mM NaF, 1 mM sodium orthovanadate, protease inhibitors, and 125 μM DPP-IV inhibitor) and quantified with the bicinchoninic acid protein assay kit (Pierce Chemical)

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Patients should contact their physician if muscle cramps are severe, frequent (occurring daily or multiple times per day), or significantly impacting quality of life and daily activities